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Updated: Jun 5, 2025
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A Protocol for Real-time 3D Single Particle Tracking
Published on: January 3, 2018
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3D real-time single particle tracking using two-photon fluorescence from bright dye-based organic nanoparticles
Marie-Charlotte Emperauger1, Eleonore Kurek2, Florian Semmer1
1Université Paris-Saclay, École Normale Supérieure Paris-Saclay, CNRS, CentraleSupélec, LuMIn, 91190 Gif-sur-Yvette, France. francois.marquier@ens-paris-saclay.fr.
Nanoscale
|December 13, 2024
Summary
Ultrabright fluorescent organic nanoparticles enable precise 3D single-particle tracking microscopy. Their stability and brightness offer new possibilities for studying dynamic cellular processes at the nanoscale.
Area of Science:
- Biophysics
- Nanotechnology
- Microscopy
Background:
- Advanced microscopy techniques are crucial for understanding cellular dynamics.
- Current tracking methods face limitations in precision, stability, and photobleaching.
Purpose of the Study:
- To evaluate ultrabright dye-based fluorescent organic nanoparticles for 3D single-particle tracking.
- To assess their suitability for long-term, high-precision biological imaging.
Main Methods:
- Utilized a 3D single-particle tracking two-photon microscopy setup.
- Characterized nanoparticles composed of quadrupolar dyes for their optical and structural properties.
Main Results:
- Demonstrated nanoparticles with large two-photon absorption cross-sections and low photobleaching.
- Achieved nanometer localization precision due to high nanoparticle brightness.
- Confirmed structural stability at physiological temperatures.
Conclusions:
- Dye-based fluorescent organic nanoparticles are effective for precise and stable nanoscale tracking.
- These nanoparticles offer advantages over inorganic nanocrystals for biological applications.
- Potential for advancing the study of dynamic cellular processes.
