Nonlinear transcriptomic responses to compounded environmental changes across temperature and resources in a pest...

Many species are experiencing drastic and multidimensional changes to their environment due to anthropogenic events. These multidimensional changes may act nonadditively on physiological and life history responses, and thus may not be predicted by responses to single dimensional environmental changes. Therefore, work is needed to understand species' responses to multiple aspects of change. We used whole-transcriptomic RNA-Sequencing and life history assays to uncover responses to singly-applied shifts in resource or temperature environmental dimensions, in comparison to combined, multidimensional change, in the crop pest seed beetle, Callosobruchus maculatus. We found that multidimensional change caused larger fecundity, developmental period and offspring viability life history changes than predicted by additive effects of 1-dimensional changes. In addition, there was little overlap between genes differentially expressed under multidimensional treatment versus under altered resource or temperature conditions alone. Moreover, 115 genes exhibited significant resource × temperature interaction effects on expression, including those involved in energy metabolism, detoxification, and enhanced formation of cuticle structural components. We conclude that single dimensional changes alone cannot determine life history and transcriptomic responses to multidimensional environmental change. These results highlight the importance of studying multidimensional environmental change for understanding the molecular and phenotypic responses that may allow organisms including insects to rapidly adapt simultaneously to multiple aspects of environmental change.