Related Experiment Video
Updated: Jul 6, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Genomic Comparison of Reoccurring, Emerging, and Persistent (REP) Shiga Toxin-Producing Escherichia coli O157:H7
Yishan Yang1, Xianghe Yan1, Bradd J Haley1
1Environmental Microbial and Food Safety Laboratory, Agricultural Research Service-US Department of Agriculture (ARS-USDA), Beltsville Agricultural Research Center, Beltsville, Maryland, USA.
Abstract:
Escherichia coli O157:H7 strains associated with several recent (2017-2020) multi-state outbreaks linked to leafy green vegetables have been characterized as "reoccurring, emerging, and persistent" (REP). Our recent unpublished work demonstrated that the REP strains had significantly enhanced potential for biofilm formation. In this study, comparative genomic analyses were conducted for a better understanding of the mechanisms behind the enhanced biofilm formation, and thereby potentially increased environmental fitness, by the REP strains. Phylogenetically, the recent outbreak strains formed two distinct clusters represented by REPEXH01 and REPEXH02. Compared with EDL933 and other previous outbreak reference strains, the REP strains (clustering with REPEXH02) exhibiting strong biofilm formation were found to have acquired two genes encoding proteins of unknown functions (hypothetical proteins) and lost certain prophage-related genes. In addition, several single nucleotide polymorphisms in genes related to biofilm formation were identified.
More Related Videos
09:26Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Related Concept Videos
Stringent Response in E. coli
Modern Molecular Taxonomy
Evolution of Microbial Genome
Bacterial Gastroenteritis