Related Experiment Video
Updated: Jun 13, 2025
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Published on: December 26, 2012
Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs
Lulu Huang1, Amy Tang1, Bojing Shao1
1Laboratory of Vascular Inflammation and Thrombosis Research, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10065, USA.
Abstract:
Currently, the only approach for obtaining alveolar epithelial type I (AT1) cells is through the differentiation of cultured AT2 cells, which has several limitations. Here, we present a protocol to prepare AT1 cells directly from mouse lungs. We detail the steps for dissociating epithelial cells from the alveoli, followed by the purification of AT1 cells. Additionally, we describe how to assess the purity of AT1 cells using immunofluorescence staining and flow cytometry, along with approaches for verifying their functions.
