A stand-alone and point-of-care electrochemical immuno-device for Salmonella typhimurium testing

The rapid development of accurate and point-of-care diagnostic tools for foodborne diseases has made a massive impact in global health. Salmonella typhimurium (S. typhimurium) exemplifies an enteric pathogen, being a gram-negative bacteria responsible for several gastrointestinal and systemic illnesses. However, the existing electrochemical devices used to detect S. typhimurium have always been bulky or unfully integrated, implying a critical need for the design and development of stand-alone and point-of-care electrochemical sensors for portable S. typhimurium testing. Herein, we present the first instance of a stand-alone and point-of-care electrochemical immuno-device (SPEID) capable of conducting S. typhimurium analysis in actual specimens of purified drinking water that is mixed with S. typhimurium and watermelon juice that is mixed with S. typhimurium at point-of-care. The development of SPEID for S. typhimurium testing is achieved by overcoming substantial engineering challenges in seamlessly integrating an autonomous-transportation module (ATM) for microfluidic autonomous and directional liquid transportation, an immune-testing module (ITM) for S. typhimurium testing, and an electronic-integration module (EIM) for converting signal and wirelessly transmitting. The SPEID is a stand-alone one which possesses pump-free and cost-effective feature for measuring S. typhimurium at point of care.