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Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
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The CALERIE Genomic Data Resource
C P Ryan1, D L Corcoran2, N Banskota3
1Robert N. Butler Columbia Aging Center, Columbia University Mailman School of Public Health, New York, NY, USA. cpr2139@cumc.columbia.edu.
Nature Aging
|December 13, 2024
Summary
Caloric restriction (CR) in humans shows potential to slow aging, similar to animal studies. This study provides comprehensive genomic data from the CALERIE trial to explore the molecular basis of CR
Area of Science:
- Gerontology
- Genomics
- Molecular Biology
Background:
- Caloric restriction (CR) is known to slow biological aging and extend lifespan in model organisms.
- The CALERIE trial provided evidence that CR may have similar effects in humans.
- Understanding the molecular mechanisms of CR in humans is crucial for aging research.
Purpose of the Study:
- To generate a comprehensive genomic dataset from the CALERIE trial.
- To investigate the molecular pathways and biological processes affected by CR in humans.
- To create a valuable resource for translational geroscience research.
Main Methods:
- Generated whole-genome SNP genotypes from 218 participants.
- Collected longitudinal DNA methylation, mRNA, and small RNA data at three time points.
- Utilized blood, skeletal muscle, and adipose tissue samples (n=2,327 total samples).
Main Results:
- Created a multi-tissue, multi-omics, longitudinal genomic data resource.
- The data captures molecular changes associated with CR in humans.
- This resource supports further investigation into CR's impact on aging biology.
Conclusions:
- The CALERIE Genomic Data Resource advances our understanding of CR's effects on human aging.
- This data facilitates translational geroscience by providing molecular insights.
- The resource is available for further research through the Aging Research Biobank.
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