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Abrogation of macrophage-mediated suppression of T-lymphocyte proliferation with hemoglobin and hemin is a function
Hemoglobin, hemin, and ferric ion (Fe) were shown to reverse peritoneal exudate cell (PEC)-mediated suppression of concanavalin A-elicited murine spleen cell activation. Titration of hemoglobin and hemin relative to Fe showed a direct relationship between Fe content and reversal of PEC suppression. Indomethacin enhanced the capacity of all three compounds to abrogate PEC suppression on the order of five- to eight-fold. The capacity of endogenous Fe-containing substances as hemoglobin and its catabolites, e.g., hemin, to modulate macrophage expression may be of special significance at sites of inflammation.
Hemoglobin, hemin, and ferric ion (Fe) were shown to reverse peritoneal exudate cell (PEC)-mediated suppression of concanavalin A-elicited murine spleen cell activation. Titration of hemoglobin and hemin relative to Fe showed a direct relationship between Fe content and reversal of PEC suppression. Indomethacin enhanced the capacity of all three compounds to abrogate PEC suppression on the order of five- to eight-fold. The capacity of endogenous Fe-containing substances as hemoglobin and its catabolites, e.g., hemin, to modulate macrophage expression may be of special significance at sites of inflammation.