Isolation of 5-hydroxy-7-methoxy-8-O-β-D-glucopyranosyl-2S-flavanone from a new source, Haplanthodes tentaculatus:...

This study explores the anticancer properties of Haplanthodes tentaculatus, a plant indigenous to the Western Ghats of India. We isolated and characterised 5-hydroxy-7-methoxy-8-O-β-D-glucopyranosyl-2S-flavanone from H. tentaculatus, previously unreported in this plant, using preparative HPLC, NMR, and HRMS techniques. The compound's molecular structure and purity were confirmed, and its biological activity was evaluated on the MDA-MB-231 breast cancer cell line using the MTT assay. The compound exhibited notable cytotoxic effects with an IC₅₀ value of 155.21 ± 1.59 sM. This work validates the therapeutic capabilities of H. tentaculatus and contributes to ongoing research on natural substances for anticancer drug development. The results suggest that H. tentaculatus could be a promising source of bioactive compounds, opening new possibilities for cancer therapy approaches.