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Updated: Jun 5, 2025

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
GhRAP2.4 enhances drought tolerance by positively regulating the strigolactone receptor GhD14 expression in cotton
Jie Dong1, Cong Ding1, Huahui Chen1
1College of Agronomy, Shandong Agricultural University, No. 61 Daizong Street, Tai'an, Shandong 271018, People's Republic of China.
Abstract:
Drought poses significant challenges to crop productivity, necessitating a deeper understanding of plant adaptive mechanisms. Strigolactones (SLs), a class of phytohormones, have been recognized as crucial regulators in plant responses to drought, yet the specific role of SL receptor in drought tolerance in cotton (Gossypium hirsutum L.) remains underexplored. In this study, we identified and characterized DWARF14 (GhD14), a SL receptor in cotton. Silencing GhD14 in cotton compromised drought tolerance by reducing leaf relative water content and chlorophyll content, slowing stomatal closure, increasing reactive oxygen species levels, and decreasing antioxidant enzyme activities. Conversely, overexpression of GhD14 in Arabidopsis d14 mutants rescued their drought-sensitive phenotype. Further, we identified a 197-bp fragment (-697 to -894 bp) in the GhD14 promoter that plays a crucial role in the drought stress response. RELATED TO APETALA 2.4 (GhRAP2.4), a dehydration responsive element binding protein (DREB) transcription factor, binds directly to the GhD14 promoter, enhancing its transcription under drought conditions. Silencing GhRAP2.4 in cotton resulted in reduced drought tolerance. This study not only elucidates the molecular interplay between GhRAP2.4 and GhD14 in cotton's drought response but also provides a potential target for genetic modification to improve drought resilience in crops.
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