Rationalizing hydrogel-integrated peroxidase-mimicking nanozymes for combating drug-resistant bacteria and...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Enzyme Mimics

Background:

• Nanozymes offer advantages over natural enzymes but face challenges in biotoxicity and bioavailability.
• Carrier systems are crucial for enhancing nanozyme performance in biomedical applications.
• Systematic studies on nanozyme@carrier platforms are limited.

Purpose of the Study:

• To optimize hydrogel carriers for peroxidase (POD)-mimic nanozymes.
• To investigate the structure-function relationship between nanozymes and carriers.
• To develop an integrated nanoplatform for disinfection and biomedical sensing.

Main Methods:

• Synthesized five hydrogel types: sodium alginate (SA), chitosan, gelatin, GelMA, and PAM.
• Incorporated a hemin and BSA-based POD-mimic nanozyme into hydrogel carriers.
• Evaluated nanozyme catalytic activity, antibacterial effects, and H2O2 sensing within different hydrogel platforms.

Main Results:

• SA hydrogel demonstrated superior compatibility and stability for nanozyme loading and function.
• Nanozyme-loaded SA hydrogel exhibited enhanced catalytic performance.
• Improved antibacterial efficacy and sensing ability for H2O2 detection were observed.

Conclusions:

• Sodium alginate hydrogels are optimal carriers for POD-mimic nanozymes, preserving their activity.
• The developed nanozyme@SA hydrogel platform shows promise for environmental disinfection and biomedical sensing.
• Understanding carrier-nanozyme interactions is key to designing advanced nanoplatforms.