Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Complementary DNA

Complementary DNA

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genomic insights into specialized and primary metabolism gene clusters in methicillin-resistant Staphylococcus aureus from Egyptian community settings.

BMC genomics·2026

Same author

Evaluation of the accuracy of a custom-made plate in maxillary repositioning.

Archives of craniofacial surgery·2026

Same author

Prophage genomics of carbapenemase-producing Klebsiella pneumoniae from animal-derived food sources.

Scientific reports·2026

Same author

SIRT7 regulates dosage compensation and safeguards the female X chromosome.

Nature·2026

Same author

Advancing One Health genomics in Africa: opportunities and challenges for outbreak and antimicrobial resistance control.

Clinical microbiology reviews·2026

Same author

Targeting Gut Microbiota by DPP-4 Inhibitors in Obesity: Mechanistic Insights and Therapeutic Implications.

Current nutrition reports·2026

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Insights into the human cDNA: A descriptive study using library screening in yeast.

Zina Alaswad¹, Nayera E Attallah¹, Basma Aboalazm¹

¹Center for Genomics, Helmy Institute for Medical Sciences, Zewail City of Science and Technology, Giza, Egypt; University of Science and Technology, Zewail City of Science and Technology, Giza, Egypt.

Journal, Genetic Engineering & Biotechnology

|December 14, 2024

Summary

Human cDNA library screening in yeast identifies gene functions but faces challenges. Many false positives arise from mutations, and transcripts often lack necessary fusion for yeast two-hybrid screening, necessitating optimization.

Keywords:

Genetic screens Saccharomyces cerevisiae Yeast two-hybrid cDNA library screens

More Related Videos

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Mating-based Overexpression Library Screening in Yeast

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

Related Experiment Videos

Last Updated: Jun 18, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Mating-based Overexpression Library Screening in Yeast

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Human cDNA libraries in yeast facilitate gene function discovery and interaction partner identification.
Techniques include yeast two-hybrid (Y2H) and classical cDNA library screens.

Purpose of the Study:

To summarize challenges encountered when implementing human cDNA library screens in Saccharomyces cerevisiae (budding yeast).
To gain insights into transcription products and highlight difficulties in cDNA screening.

Main Methods:

Utilized DNA repair-deficient yeast strains for drug toxicity rescue screens.
Employed Sanger sequencing to analyze 282 transcripts from various yeast screens and a human kidney cDNA library.

Main Results:

Observed numerous transcripts rescuing toxic effects, but most were false positives due to spontaneous yeast mutations.
Identified issues like mitochondrial transcripts, non-coding RNAs, truncated cDNAs, and lack of GAL4 activation domain (GAL4AD) fusion for Y2H.
Sanger sequencing revealed diverse transcription products and screening inefficiencies.

Conclusions:

Human cDNA library screening in yeast presents significant challenges, including high false positive rates and unsuitable transcript formats.
Despite difficulties, the technique can identify important molecular mechanisms, indicating a need for process optimization to enhance efficiency.