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Recovery of ergosterol from Agaricus bisporus mushrooms via supercritical fluid extraction: A response surface
Cláudia F Almeida1,2,3, Yaidelin A Manrique1,3, José Carlos B Lopes1,3
1LSRE-LCM - Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Abstract:
This work uses the response surface methodology to optimise ergosterol recovery from Agaricus bisporus mushrooms by supercritical fluid extraction. The influence of pressure, temperature, co-solvent use, and solvent-to-mushroom mass ratio was evaluated on yield and extract composition. Considering temperature, pressure, and co-solvent volume percentage, predictive models were regressed for extraction yield, ergosterol purity in the recovered fractions, and ergosterol recovery. The co-solvent volumetric fraction was the most influential factor, increasing the extraction yield and reducing the extract's ergosterol purity. A maximum ergosterol extract purity of (547.27 ± 2.37) was obtained at a pressure of 100 bar, temperature of 50 °C and co-solvent volume percentage of . The highest ergosterol recovery was (6.23 ± 0.06) for operating conditions of 244 bar, °C, and of co-solvent. Coefficients of determination close to one were obtained for all models, indicating good agreement with experimental data.
