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Genetically determined molecular weight differences in murine complement component C6.

A Orren, C J Preece, E B Dowdle

European Journal of Immunology

|January 1, 1985

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CBA mice possess two forms of complement component 6 (C6), types A and B, while BALB/c and DBA/2 mice only have type A. The high molecular weight type B C6 is an autosomal dominant trait.

Area of Science:

Immunogenetics
Complement System Biology
Biochemistry

Background:

Complement component 6 (C6) is crucial for the formation of the membrane attack complex.
Genetic variations in complement proteins can impact immune responses and disease susceptibility.
Previous studies have identified different molecular forms of complement proteins in various species.

Purpose of the Study:

To investigate the molecular heterogeneity of complement component 6 (C6) in different mouse strains.
To characterize the biochemical properties and inheritance patterns of C6 variants.
To establish a genetic basis for C6 polymorphism in mice.

Main Methods:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to separate C6 by molecular weight.

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Erythrocyte/agarose overlay gels to detect hemolytic activity of C6 bands.

Flatbed isoelectric focusing (IEF) to determine C6 charge variants.

Functional assays to confirm hemolytic activity of separated C6 forms.

Cross-breeding experiments to analyze inheritance patterns.

Main Results:

CBA mouse plasma exhibited two C6 forms: type A (Mr ~90,000) and type B (Mr ~100,000).
BALB/c and DBA/2 mouse plasma contained only type A C6.
Isoelectric focusing revealed distinct pI values for type A and type B C6, with CBA plasma showing two hemolytic band sets.
Breeding experiments indicated that the high molecular weight type B C6 is inherited as an autosomal dominant trait.

Conclusions:

Mouse complement component 6 (C6) exists in at least two molecular forms (A and B) with distinct molecular weights and isoelectric points.
The presence of high molecular weight type B C6 is controlled by an autosomal dominant gene.
This genetic polymorphism in C6 may have implications for understanding immune system variation in mice.