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  1. Home
  3. Investigating Angiogenesis-related Biomarkers In Osteoarthritis Patients Through Transcriptomic Profiling.
  1. Home
  3. Investigating Angiogenesis-related Biomarkers In Osteoarthritis Patients Through Transcriptomic Profiling.

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Investigating Angiogenesis-Related Biomarkers in Osteoarthritis Patients Through Transcriptomic Profiling.

Yang Zheng1, Miaojia Fang2, Shriya Sanan1

  • 1Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, People's Republic of China.

Journal of Inflammation Research
|December 16, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

This study identifies key angiogenesis-related genes (ARGs) like COL3A1 and OLR1 as potential biomarkers for osteoarthritis (OA). These findings may lead to new diagnostic tools and therapeutic targets for OA progression.

Keywords:
angiogenesisbiomarkerimmune infiltrationosteoarthritisregulatory network

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Area of Science:

  • Biomedical research
  • Genomics
  • Molecular biology

Background:

  • Osteoarthritis (OA) is a prevalent degenerative joint disease impacting quality of life.
  • Angiogenesis, the formation of new blood vessels, is a critical factor in OA pathogenesis.
  • Identifying key genes involved in angiogenesis is crucial for understanding OA progression.

Purpose of the Study:

  • To identify key angiogenesis-related genes (ARGs) implicated in osteoarthritis (OA) using transcriptomic and machine learning approaches.
  • To evaluate the diagnostic potential of identified ARGs in OA.
  • To explore potential therapeutic targets and pathways associated with OA angiogenesis.

Main Methods:

  • Analysis of differentially expressed genes (DEGs) in OA synovial tissues.
  • Integration of Weighted Gene Co-expression Network Analysis (WGCNA) with ARGs.
  • Application of machine learning algorithms and ROC curve analysis for candidate gene selection.
  • Gene Set Enrichment Analysis (GSEA) and immune cell infiltration analysis.
  • RT-qPCR validation of identified biomarkers in clinical samples.

    • Main Results:

    • Four key angiogenesis-related genes (COL3A1, OLR1, STC1, KCNJ8) demonstrated significant diagnostic value in OA.
    • These biomarkers were associated with the lysosome pathway and specific immune cells (eosinophils, Th2 cells).
    • COL3A1, OLR1, and STC1 expression levels were validated in clinical synovial samples, confirming their role in OA.

    Conclusions:

    • COL3A1, OLR1, STC1, and KCNJ8 are identified as crucial angiogenesis-related biomarkers for osteoarthritis.
    • These genes represent potential diagnostic tools and therapeutic targets for OA.
    • Targeting angiogenesis pathways offers promising new strategies for osteoarthritis treatment.