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Related Experiment Video

Updated: Jun 5, 2025

Author Spotlight: Developing Multiplexed Kinetic Assays for Organoid-Based Drug Response Analysis

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Published on: January 5, 2024

Recent advances in SERS-based bioanalytical applications: live cell imaging.

Dong-Kwon Lim^1,2,3, Panangattukara Prabhakaran Praveen Kumar¹

¹KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.

Nanophotonics (Berlin, Germany)

|December 16, 2024

Summary

Surface-enhanced Raman scattering (SERS) offers sensitive, high-resolution live cell imaging, overcoming limitations of fluorescence methods. This review highlights SERS advancements for cellular analysis, drug screening, and real-time monitoring.

Keywords:

Raman scattering Stokes-scattering live cellular imaging molecular signal single cell analysis surface-enhanced Raman scattering

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Area of Science:

Spectroscopy and imaging
Nanobiotechnology
Material Science

Background:

Raman scattering provides molecular fingerprints, valuable in material science and nanobiotechnology.
Its low interference with water makes it suitable for biological applications like live cell imaging.
Conventional fluorescence methods suffer from photobleaching and lack molecular specificity.

Purpose of the Study:

To review recent advances in Surface-Enhanced Raman Scattering (SERS) for live cell imaging.
To explore SERS applications in drug screening, cellular signaling, and in situ sensing.
To highlight the role of nanostructures and surface chemistry in enhancing SERS performance.

Main Methods:

Utilizing plasmonic nanomaterials to amplify the weak Raman scattering signal.
Developing novel nanostructure designs and surface chemistries for targeted sensing.
Employing advanced optical setups for high spatiotemporal resolution imaging.

Main Results:

SERS enables molecular-specific information acquisition with high sensitivity and resolution.
Demonstrated potential for real-time monitoring of cellular processes and drug responses.
Achieved non-damaging, high-resolution imaging of live cells.

Conclusions:

SERS is a powerful, promising tool for live cell imaging, surpassing conventional methods.
Advancements in nanostructures and optical setups are crucial for practical SERS applications in cell biology.
SERS holds significant potential for drug screening and understanding cellular dynamics.