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Prodigiosin hydrogel to promote healing of trauma-infected multidrug-resistant Staphylococcus aureus mice wounds
Xin Wang1, Guangfan Meng1, Zongyu Zhang1
1School of Bioengineering, State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Abstract:
Wound infections caused by Multidrug-resistant Staphylococcus aureus (MRSA) have been regarded as a challenging problem in clinic for the long time. In this study, based on the excellent antimicrobial effect of prodigiosin(PG) and the ability of hydrogel dressing in terms of tissue repair and regeneration, we prepared the PG hydrogel as a treatment for the wound infection induced by MRSA. Rheological tests indicated that PG hydrogel as a semi-solid gel had good mechanical properties. In ex vitro drug permeation studies and dermatokinetic studies showed that PG hydrogel had high PG permeability and were capable of short-term retention in the skin. In addition, in vivo experiments for mouse skin wounds showed that the serum levels of inflammatory factors including IL-β and other inflammatory factors were reduced, the inflammatory infiltration of tissues was reduced, the transcript levels of genes such as COL1A1 were up-regulated at different stages of wound healing, and the relative abundance of genera such as Desulfovibrio was lowered after treatment with PG hydrogel, which facilitated wound healing in mice. Our study would provide a new solution to the clinical shortage of drugs for the treatment of MRSA infection and provide a research basis for improving the comprehensive values of PG.
Insights
This study developed a prodigiosin (PG) hydrogel to treat wound infections from multidrug-resistant Staphylococcus aureus (MRSA). The PG hydrogel demonstrated effective antimicrobial properties and promoted wound healing in mice.
Area of Science:
- Biomaterials Science
- Infectious Diseases
- Wound Healing
Background:
- Multidrug-resistant Staphylococcus aureus (MRSA) poses a significant clinical challenge for wound infections.
- Existing treatments for MRSA infections are limited, necessitating novel therapeutic approaches.
Purpose of the Study:
- To develop and evaluate a novel prodigiosin (PG) hydrogel for treating MRSA-induced wound infections.
- To assess the efficacy of PG hydrogel in promoting tissue repair and regeneration in infected wounds.
Main Methods:
- Preparation and characterization of PG hydrogel, including rheological and mechanical property testing.
- In vitro drug permeation and dermatokinetic studies to evaluate PG release and skin retention.
- In vivo studies on mouse skin wounds to assess inflammatory markers, gene expression, and microbial changes.
Main Results:
- PG hydrogel exhibited favorable mechanical properties and demonstrated high PG permeability with short-term skin retention.
- In vivo experiments showed reduced serum inflammatory factors (e.g., IL-1β), decreased tissue inflammatory infiltration, and up-regulation of collagen gene (COL1A1) expression.
- Treatment with PG hydrogel led to a reduction in the abundance of specific bacterial genera, such as Desulfovibrio, facilitating wound healing.
Conclusions:
- PG hydrogel is a promising therapeutic agent for MRSA-induced wound infections, offering both antimicrobial effects and enhanced wound healing.
- This study provides a foundation for utilizing prodigiosin in advanced wound care and addressing the clinical need for effective MRSA treatments.
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