Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein synthesis, thermotolerance and step down heating.

G M Hahn, E C Shiu

    International Journal of Radiation Oncology, Biology, Physics
    |January 1, 1985
    PubMed
    Summary

    Mammalian cells develop heat resistance (thermotolerance) through protein synthesis. Step-down heating (SDH) sensitization occurs when protein synthesis is severely inhibited, suggesting SDH arises from inhibited thermotolerance development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Increase in radiation-induced HPRT gene mutation frequency after nonthermal exposure to nonionizing 60 Hz electromagnetic fields.

    Radiation research·1999
    Same author

    Stable overexpression of human HSF-1 in murine cells suggests activation rather than expression of HSF-1 to be the key regulatory step in the heat shock gene expression.

    Journal of cellular biochemistry·1995
    Same author

    Modulation of potassium channels by protein tyrosine kinase inhibitors.

    Journal of cellular physiology·1994
    Same author

    Correlation of heat resistance and HSP-70A mRNA levels in human tumor cells measured by competitive quantitative polymerase chain reaction.

    International journal of radiation oncology, biology, physics·1994
    Same author

    Mutagenesis in mammalian cells can be modulated by radiation-induced voltage-dependent potassium channels.

    Mutation research·1994
    Same author

    Formation of tight junctions and desmosomes protects MDCK cells against hyperthermic killing.

    Journal of cellular physiology·1994

    Area of Science:

    • Cellular biology
    • Molecular biology
    • Biophysics

    Background:

    • Mammalian cell heat sensitivity depends on prior thermal exposure.
    • Thermotolerance (TT) involves resistance to subsequent heat stress after mild heating.
    • Step-down heating (SDH) causes increased sensitivity after initial exposure, but its molecular basis is unknown.

    Purpose of the Study:

    • Investigate the relationship between the rate of protein synthesis (rPS) and both thermotolerance (TT) and step-down heating (SDH) in HA1 cells.
    • Elucidate the molecular events underlying SDH and its connection to TT.

    Main Methods:

    • Exposure of HA1 cells to different temperature sequences (mild heat followed by higher heat for TT, or higher heat directly for SDH).
    • Measurement of the rate of protein synthesis (rPS) under various thermal conditions.
    • Analysis of rPS recovery during and after heat exposure.

    Main Results:

    • TT cells exhibited a 30% higher rPS than unheated cells, with only transient rPS reductions up to 43°C.
    • SDH sensitization occurred in unheated cells only with severe, prolonged rPS inhibition (<10% of control).
    • TT cells did not exhibit SDH and showed transient rPS reductions, unlike unheated cells.

    Conclusions:

    • Protein synthesis is essential for the development of thermotolerance (TT).
    • Step-down heating (SDH) sensitization is primarily a consequence of the inhibition of TT development.
    • The rate of protein synthesis is a critical factor modulating cellular responses to thermal stress.

    Related Experiment Videos