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Vaccinations01:51

Vaccinations

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Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
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Tumorigenicity decrease in Bcl-xL deficient MDCK cells ensuring the safety for influenza vaccine production.

Jiahao Zheng1,2, Boran Li3, Lanxin Jia1,2

  • 1National Engineering Technology Research Center for Combined Vaccines, Wuhan, China.

Plos One
|December 16, 2024
PubMed
Summary
This summary is machine-generated.

Researchers modified Madin-Darby canine kidney (MDCK) cells to create the BY-02 cell strain, significantly reducing tumorigenicity for safer influenza vaccine production. This engineered cell line shows reduced proliferation and increased apoptosis, enhancing vaccine safety.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Vaccinology

Background:

  • Madin-Darby canine kidney (MDCK) cells are standard for influenza vaccine manufacturing.
  • The inherent tumorigenic potential of MDCK cells poses a safety concern for biological product development.

Purpose of the Study:

  • To engineer a safer MDCK cell line for influenza vaccine production by reducing tumorigenicity.
  • To assess the impact of B-cell lymphoma extra-large (Bcl-xL) gene knockout on MDCK cell characteristics and tumor formation.

Main Methods:

  • CRISPR-Cas9 gene editing was employed to knock out the Bcl-xL gene in MDCK cells, generating the BY-02 cell strain.
  • Comparative analysis of proliferation, migration, apoptosis, and mitochondrial pathways between original MDCK and BY-02 cells.
  • Tumor formation assays were conducted in nude mice using both cell types.

Main Results:

  • The BY-02 cell strain exhibited significantly reduced proliferation and migration compared to original MDCK cells.
  • Apoptosis levels were significantly increased in BY-02 cells, with modulation of the endogenous mitochondrial apoptotic pathway.
  • Tumor formation assays demonstrated a substantial decrease in tumor incidence in mice injected with BY-02 cells versus MDCK cells.

Conclusions:

  • Knocking down the Bcl-xL gene effectively inhibits tumor formation in MDCK cells.
  • The genetically engineered BY-02 cell strain is a promising candidate for enhancing the safety of influenza vaccine production.