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Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Antibiotic Selection00:57

Antibiotic Selection

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Overview
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  1. Home
  3. Field-based Evidence For The Prevalence Of Soil Antibiotic Resistomes Under Long-term Antibiotic-free Fertilization.
  1. Home
  3. Field-based Evidence For The Prevalence Of Soil Antibiotic Resistomes Under Long-term Antibiotic-free Fertilization.

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Field-based evidence for the prevalence of soil antibiotic resistomes under long-term antibiotic-free fertilization.

Yuhao Fu1, Fang Hu2, Fang Wang1

  • 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Environment International
|December 16, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Antibiotic-free manure reduces soil antibiotic resistance genes (ARGs) compared to chemical fertilizer. However, combined application and other factors still contribute to ARG prevalence, necessitating comprehensive agricultural strategies.

Keywords:
Antibiotic resistomeAntibiotic-free manureBacterial communityBlack soilLong-term fertilization

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Area of Science:

  • Environmental microbiology
  • Soil science
  • Agricultural science

Background:

  • Antibiotic residues in manure increase soil antibiotic resistance genes (ARGs).
  • Limited understanding exists regarding long-term effects of antibiotic-free fertilization on soil ARGs.

Purpose of the Study:

  • Quantify antibiotic resistome assembly patterns under long-term antibiotic-free manure and chemical fertilizer treatments.
  • Investigate the influence of fertilization types on bacterial communities and environmental factors affecting soil ARGs.

Main Methods:

  • In situ probing of bacterial communities and environmental variations in field soils.
  • Analysis of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) abundances.
  • Statistical analysis of factors influencing ARG assembly.

Main Results:

  • Long-term fertilization slightly impacted ARG diversity, with 85.5% of ARGs and MGEs common across treatments.
  • Antibiotic-free manure resulted in less pronounced ARG abundance increases than chemical fertilizer.
  • Combined application of manure and chemical fertilizer significantly increased nutrients, MGEs, and ARGs, particularly Rank I and II ARGs.

Conclusions:

  • Antibiotic-free manure can lower ARG risks but does not eliminate them.
  • Mobile genetic elements (MGEs) significantly shape ARGs through direct and indirect pathways.
  • Comprehensive strategies beyond antibiotic prohibition are needed to address agricultural antibiotic resistance.