Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

202
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
202
One-Compartment Open Model for Extravascular Administration: First-Order Absorption Model01:15

One-Compartment Open Model for Extravascular Administration: First-Order Absorption Model

192
The first-order absorption model for extravascular administration describes the rate at which a drug is absorbed and eliminated, following the principles of first-order kinetics. This model is vital as it provides a mathematical representation of drug behavior within the body. It also allows for the prediction and interpretation of drug absorption and elimination based on the rate of change in drug concentration over time. This model can be visualized as a plasma concentration-time profile...
192
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

202
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
202
Two-Compartment Open Model: Extravascular Administration01:12

Two-Compartment Open Model: Extravascular Administration

151
The two-compartment model for extravascular administration represents a drug's absorption and distribution process. It features a central compartment, where the drug is first absorbed, and a peripheral compartment, which illustrates the drug's distribution throughout the body. The rate of change in drug concentration in the central compartment is calculated by three exponents: absorption, distribution, and elimination.
The absorption exponent (ka) indicates the speed at which the drug...
151
One-Compartment Open Model for Extravascular Administration: Zero-Order Absorption Model01:12

One-Compartment Open Model for Extravascular Administration: Zero-Order Absorption Model

60
Extravascular administration, such as oral or intramuscular routes, is a non-invasive drug delivery method, often preferred for ease and patient compliance. A key factor here is absorption, which dictates how quickly and effectively the drug enters the bloodstream from the administration site. Absorption follows either zero-order or first-order kinetics.
Zero-order absorption maintains a steady rate irrespective of the amount of drug left to be absorbed, making it a constant process. In the...
60
Model Approaches for Pharmacokinetic Data: Compartment Models01:14

Model Approaches for Pharmacokinetic Data: Compartment Models

75
Compartmental analysis is a widely adopted approach to characterizing drug pharmacokinetics. It uses compartment models that conceptualize the body as a collection of reversibly communicating compartments, each representing a group of tissues exhibiting similar drug distribution characteristics. The movement rate of the drug between these compartments is typically described by first-order kinetics.
Two primary types of compartment models are recognized: mammillary and catenary. The more...
75

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation and Properties of Magnetically Responsive Graphene/Boron Nitride/Iron Oxide Filler Composite Epoxy Resin Materials.

Nanomaterials (Basel, Switzerland)·2025
Same author

Deep brain stimulation for dystonia treatment in cerebral palsy: efficacy exploration.

Experimental biology and medicine (Maywood, N.J.)·2025
Same author

Advanced glycation endproducts induce cytokine dysregulation and weaken lung epithelial and endothelial barrier integrity.

Tissue barriers·2025
Same author

From transmission to adaptive evolution: genomic surveillance of Getah virus.

Frontiers in cellular and infection microbiology·2025
Same author

Correction to "<i>ortho</i>-C-H Amidation of Phenols through Intramolecular Rearrangement of <i>N</i>-Phenoxyarylamides".

Organic letters·2025
Same author

<i>Bandavirus dabieense</i>: A review of epidemiology, clinical characteristics, pathophysiology, treatment and prevention.

Virulence·2025

Related Experiment Video

Updated: Jun 5, 2025

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

25.2K

A computational workflow for end-to-end simulation of percutaneous absorption.

Duo Zhang1, Benjamin N Deacon1, Weijun Li1

  • 1School of Chemistry and Chemical Engineering, University of Surrey, Guildford GU2 7XH, UK.

International Journal of Pharmaceutics
|December 16, 2024
PubMed
Summary

A new workflow enables end-to-end in silico modeling of dermal absorption, creating a digital twin for non-experts. This tool accurately predicts percutaneous absorption, including formulation and evaporation effects.

More Related Videos

Author Spotlight: Computing the Effects of a Local Radiofrequency Hyperthermia Intervention on Tumor Biomechanics
10:23

Author Spotlight: Computing the Effects of a Local Radiofrequency Hyperthermia Intervention on Tumor Biomechanics

Published on: December 1, 2023

361
Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
11:07

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

2.7K

Related Experiment Videos

Last Updated: Jun 5, 2025

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

25.2K
Author Spotlight: Computing the Effects of a Local Radiofrequency Hyperthermia Intervention on Tumor Biomechanics
10:23

Author Spotlight: Computing the Effects of a Local Radiofrequency Hyperthermia Intervention on Tumor Biomechanics

Published on: December 1, 2023

361
Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
11:07

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

2.7K

Area of Science:

  • Computational toxicology
  • Pharmacokinetics
  • Dermal absorption modeling

Background:

  • In silico modeling offers a powerful alternative to traditional in vitro and in vivo methods for assessing dermal absorption.
  • Developing user-friendly tools is crucial for broader adoption by non-specialists in regulatory and research settings.

Purpose of the Study:

  • To develop an automated, end-to-end workflow for in silico modeling of percutaneous absorption.
  • To create a digital twin for simulating transdermal permeation accessible to non-modeling experts.

Main Methods:

  • A KNIME-based workflow integrating physicochemical property informatics, molecular dynamics, and QSPRs.
  • Physiologically based pharmacokinetic (PBPK) modeling incorporating permeant/solvent evaporation for unoccluded conditions.
  • Automated data processing, result reporting, and formulation database management.

Main Results:

  • The workflow successfully predicted percutaneous absorption, aligning well with published in vitro permeation test (IVPT) data.
  • Model predictions accurately reflected the influence of formulation vehicles and evaporation on absorption.

Conclusions:

  • The automated workflow simplifies complex in silico dermal absorption simulations for non-experts.
  • The model robustly handles various formulation and exposure conditions, including volatile compound evaporation.