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Updated: Jun 5, 2025

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
Combining quorum quenching by Rhodococcus sp. BH4 and Acinetobacter sp. DKY-1 to control biofouling in membrane
Yanyao Huang1, Xueman Zheng1, Yunshi Feng1
1Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Abstract:
This study investigates a novel approach to mitigate biofouling in membrane bioreactors (MBRs) using a combinational quorum quenching (QQ) strategy. Rhodococcus sp. BH4 and Acinetobacter sp. DKY-1 were employed to disrupt intraspecies N-acyl-homoserine lactones (AHL) and interspecies autoinducer-2 (AI-2) quorum sensing, respectively. BH4 and DKY-1 were immobilized independently and the antibiofouling effects of single QQ beads and 1: 1 mixed QQ beads, both with the same final doses, were compared. While both bead types exhibited high QQ activity, the mixed QQ beads more effectively inhibited microbial biofilm formation, delaying biofouling by two times compared to 1.5 and 1.7 times for the single species beads. Additionally, the mixed QQ MBR demonstrated significantly lower extracellular polymeric substances and a notable reduction in the genus Nitrospira. This combined QQ strategy presents a promising method for enhancing antibiofouling performance in MBRs through targeted disruption of microbial communication.
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