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Related Experiment Videos

Endothelial fenestral diaphragms: a quick-freeze, deep-etch study.

E L Bearer, L Orci

    The Journal of Cell Biology
    |February 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers visualized endothelial cell pores using quick-freezing and deep-etching. This revealed a unique structure within fenestrated capillaries responsible for transport, clarifying how substances cross these vital barriers.

    Area of Science:

    • Cell Biology
    • Physiology
    • Microscopy

    Background:

    • The transport route across endothelial cells is a long-standing question in physiology and cell biology.
    • Previous studies suggested slit-shaped pores (5.1-5.7 nm) but lacked direct visualization.
    • The fenestral diaphragm was hypothesized to house these transport channels.

    Purpose of the Study:

    • To visualize the ultrastructure of the fenestral diaphragm in fenestrated capillaries.
    • To identify the morphologic correlate of the endothelial cell transport channel.
    • To characterize the structure responsible for fenestrated capillary permeability.

    Main Methods:

    • Utilized quick-freezing and deep-etching techniques for tissue processing.
    • Applied platinum-carbon shadowing for electron microscopy of the diaphragms.

    Related Experiment Videos

  • Employed cationic ferritin as a tracer to identify binding sites.

    • Main Results:

    • Revealed the fenestral diaphragm composed of 7 nm radial fibrils forming a central mesh.
    • Demonstrated wedge-shaped channels with an average arc length of 5.46 nm.
    • Observed consistent diaphragmatic structure in various fenestrated capillaries (pancreas, adrenal, kidney) but not continuous capillaries.
    • Confirmed octagonal symmetry of the fenestral diaphragm structure.
    • Showed cationic ferritin binding to the structure within the fenestral pore.

    Conclusions:

    • The fenestral diaphragm contains the structure responsible for fenestrated capillary permeability.
    • The communicating channel through the diaphragm is wedge-shaped.
    • The new technique provides unprecedented visualization of endothelial transport structures.