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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-Performance Liquid Chromatography: Elution Process01:05

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Recent Advances in Screening and Separating Active Components From Natural Products Based on High-Speed

Xing-Cui Wang1,2, Xin-Yi Huang1,2, Fu-Xin Zhang1,2

  • 1CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory of Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China.

Journal of Separation Science
|December 16, 2024
PubMed
Summary

High-speed countercurrent chromatography (HSCC) effectively screens and separates active compounds from natural products. This review details HSCC combined with other methods for enhanced natural product discovery and large-scale preparation.

Keywords:
active compoundshigh‐speed countercurrent chromatographynatural productspost‐column activity assaypre‐column activity assay

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Area of Science:

  • Natural Product Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • Natural products are crucial for human health, potentially reducing cancer and cardiovascular disease incidence.
  • Effective techniques are needed for screening and separating bioactive compounds from natural sources.
  • High-speed countercurrent chromatography (HSCC) is a key liquid-liquid chromatography technique for natural product separation.

Purpose of the Study:

  • To review research progress on screening and separating active components from natural products using HSCC combined with other technologies.
  • To provide theoretical support for utilizing HSCC in natural active compound screening and separation.
  • To facilitate the large-scale preparation of valuable natural compounds.

Main Methods:

  • Review of literature combining HSCC with pre-column activity assays.
  • Review of literature combining HSCC with post-column activity assays.
  • Analysis of advancements in HSCC for natural product isolation.

Main Results:

  • HSCC, when coupled with activity assays, offers powerful strategies for identifying bioactive natural compounds.
  • Both pre-column and post-column assay integrations demonstrate significant utility in natural product research.
  • The reviewed methods support the efficient isolation and potential large-scale production of natural compounds.

Conclusions:

  • Combining HSCC with activity assays is a promising approach for natural product research.
  • Further development of these integrated techniques can enhance the discovery and application of natural compounds.
  • This review provides a foundation for optimizing HSCC-based separation for large-scale natural product preparation.