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[Calcifying bacteria and carbonic anhydrase].

C Billy, J Fournié, P Carpentier

    Comptes Rendus Des Seances De L'Academie Des Sciences. Serie D, Sciences Naturelles
    |June 18, 1979
    PubMed
    Summary
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    Carbonic anhydrase is not found in calcifying bacteria, but adding it boosts their growth and calcification by altering pH. This finding may explain dental plaque calcification.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Enzymology

    Background:

    • Bacteria play a crucial role in biomineralization processes.
    • Calcifying bacteria are known for their ability to precipitate minerals.
    • The role of carbonic anhydrase in bacterial calcification is not fully understood.

    Purpose of the Study:

    • To investigate the presence and activity of carbonic anhydrase in calcifying bacteria.
    • To determine the effect of exogenous carbonic anhydrase on bacterial growth and calcification.
    • To explore the potential application of these findings to dental plaque calcification.

    Main Methods:

    • Measurement of carbonic anhydrase activity in bacterial enzymatic equipment.
    • Culturing bacteria with and without the addition of carbonic anhydrase.

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  • Monitoring bacterial growth and calcification levels in culture media.
  • Assessing the impact of carbonic anhydrase on the pH of culture media.

    • Main Results:

    • Carbonic anhydrase was not detected in the enzymatic machinery of the studied bacteria.
    • Supplementation with carbonic anhydrase significantly enhanced bacterial growth.
    • Exogenous carbonic anhydrase increased the calcifying power of the bacterial cultures.
    • The enzyme's effect on pH was identified as the primary mechanism for enhanced growth and calcification.

    Conclusions:

    • Carbonic anhydrase is not an intrinsic enzyme in these calcifying bacteria.
    • External carbonic anhydrase can positively influence bacterial proliferation and mineral precipitation.
    • The enzyme's pH-modulating activity is key to its beneficial effects.
    • These findings offer insights into the biochemical mechanisms underlying dental plaque formation and calcification.