Related Experiment Video

Updated: Jun 4, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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Regulation and Pharmacology of the Cyclic GMP and Nitric Oxide Pathway in Embryonic and Adult Stem Cells

Alexander Y Kots1, Ka Bian1

  • 1Veteran Affairs Palo Alto Health Care System, US Department of Veteran Affairs, Palo Alto, CA 90304, USA.

Cells
|December 17, 2024
PubMed

Insights

The nitric oxide (NO) and cyclic GMP (cGMP) pathway significantly impacts stem cell functions like self-renewal and differentiation. Modulating this pathway offers potential for novel regenerative medicine strategies.

Area of Science:

  • Stem cell biology
  • Molecular signaling pathways

Background:

  • The nitric oxide (NO) and cyclic GMP (cGMP) pathway is crucial in cellular processes.
  • Stem cell differentiation involves dynamic changes in NO-cGMP pathway components.

Purpose of the Study:

  • To review recent advances in the role of the NO-cGMP pathway in stem cells.
  • To explore the pathway's influence on stem cell self-renewal, differentiation, and therapeutic applications.

Main Methods:

  • Literature review of studies on NO-cGMP pathway in stem cells.
  • Analysis of gene expression changes during stem cell differentiation.
  • Examination of cGMP-independent and -dependent mechanisms.

Main Results:

  • NO regulates undifferentiated stem cell self-renewal and survival via cGMP-independent pathways.
  • Natriuretic peptides promote undifferentiated stem cell growth through cGMP-mediated mechanisms.
  • Stem cell differentiation, migration, and homing are modulated by NO and natriuretic peptides.

Conclusions:

  • The NO-cGMP pathway plays a multifaceted role in stem cell biology.
  • Pharmacological targeting of the cGMP pathway can enhance stem cell-based therapies.
  • Manipulation of the cGMP pathway holds promise for regenerative medicine.
Keywords:
cGMPguanylyl cyclasenitric oxidephosphodiesterasestem cells

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