A Nucleotide-Binding Domain Leucine-Rich Repeat Gene Regulates Plant Growth and Defense Against Chewing Herbivores

Plant nucleotide-binding leucine-rich repeat immune receptor genes (NLRs) play an important role in plant defenses against pathogens, pathogenic nematodes, and piercing-sucking herbivores. However, little is known about their functions in plant defenses against chewing herbivores. Here, we identified a plasma membrane-localized coiled-coil-type NLR protein, OsPik-2-like, whose transcript levels were induced by the infestation of rice leaf folder (LF, Cnaphalocrocis medinalis) larvae, and by treatment with mechanical wounding. Knocking out OsPik-2-like in rice increased the LF-induced levels of jasmonic acid (JA) and jasmonoyl-isoleucine (JA-Ile), the activity of trypsin protease inhibitors (TrypPIs), and the basal levels of some flavonoids, which in turn decreased the performance of LF larvae. Moreover, knocking out OsPik-2-like reduced plant growth. These findings demonstrate that OsPik-2-like regulates the symbiosis between rice and LF by balancing plant growth and defense.