Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Copy Number Variation and Haplotype Analysis of 17q21.31 Reveals Increased Risk Associated with Progressive Supranuclear Palsy and Gene Expression Changes in Neuronal Cells.

Movement disorders : official journal of the Movement Disorder Society·2025
Same author

CNV-Profile Regression: A New Approach for Copy Number Variant Association Analysis in Whole Genome Sequencing Data.

bioRxiv : the preprint server for biology·2024
Same author

HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation.

Plant physiology·2023
Same author

Transcriptomic Signature of the SHATTERPROOF2 Expression Domain Reveals the Meristematic Nature of Arabidopsis Gynoecial Medial Domain.

Plant physiology·2016
Same author

SEUSS Integrates Gibberellin Signaling with Transcriptional Inputs from the SHR-SCR-SCL3 Module to Regulate Middle Cortex Formation in the Arabidopsis Root.

Plant physiology·2016

Related Experiment Video

Updated: Jun 21, 2026

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
08:05

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

17.7K

Machine Learning Inference of Gene Regulatory Networks in Developing Mimulus Seeds.

Albert Tucci1, Miguel A Flores-Vergara1, Robert G Franks1

  • 1Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.

Plants (Basel, Switzerland)
|December 17, 2024
PubMed
Summary

Flowering plant seeds drive diversification by creating reproductive barriers. This study infers gene regulatory networks in Mimulus hybrid seeds to uncover novel genetic mechanisms underlying seed development and speciation.

Keywords:
KBoostMimulus erythrantheRNA-seqRTP-STARembryoendospermhybrid seed inviabilitypostzygotic reproductive barriersseed developmentspeciation

More Related Videos

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.5K

Related Experiment Videos

Last Updated: Jun 21, 2026

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
08:05

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

17.7K
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.5K

Area of Science:

  • Evolutionary Biology
  • Plant Science
  • Genetics

Background:

  • Angiosperm seeds are key evolutionary innovations driving plant diversification.
  • Postzygotic reproductive barriers, like hybrid seed inviability, are crucial for speciation.
  • The Mimulus genus provides a model for studying seed-based reproductive isolation.

Purpose of the Study:

  • To investigate gene regulatory network (GRN) inference in developing Mimulus hybrid seeds.
  • To identify gene regulatory mechanisms critical for seed development and reproductive barriers.
  • To generate a reference set of putative gene regulations in Mimulus.

Main Methods:

  • Utilized time-series RNA-sequencing data from viable Mimulus hybrid seeds.
  • Applied two GRN inference algorithms: RTP-STAR and KBoost.
  • Analyzed transcriptomic data subsets to infer gene regulatory relationships.

Main Results:

  • Successfully inferred GRNs from Mimulus hybrid seed transcriptomic data.
  • Identified overlapping and distinct gene regulations between the two algorithms.
  • Highlighted potential novel regulatory mechanisms involved in seed development.

Conclusions:

  • GRN inference is a powerful approach for uncovering gene regulatory mechanisms in plant seeds.
  • The study provides insights into the genetic basis of reproductive barriers in Mimulus.
  • Identified putative gene regulations warrant further investigation for their role in speciation.