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Core-Shell Chitosan Particles Targeting Membrane-Bound Heat Shock Protein 70 for Cancer Therapy

Elena V Svirshchevskaya1, Valentina V Kostenko1, Anna A Boyko1

  • 1Laboratory of Cell Interactions, Department of Immunology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia.

Nanomaterials (Basel, Switzerland)
|December 17, 2024
PubMed

Insights

Researchers developed targeted anti-cancer nanoparticles using a monoclonal antibody against cell membrane-exposed heat shock protein 70 kDa (mHSP70). These mHSP70-targeting nanoparticles show promise for delivering anti-cancer drugs specifically to tumor cells.

Area of Science:

  • Oncology
  • Nanotechnology
  • Immunology

Background:

  • Targeted anti-cancer therapy requires identifying tumor-specific molecules.
  • Cell membrane-exposed heat shock protein 70 kDa (mHSP70) is over-expressed on various cancer cells.

Purpose of the Study:

  • To analyze mHSP70 expression on diverse tumor cells.
  • To engineer mHSP70-targeting chitosan-based nanoparticles for drug delivery.

Main Methods:

  • Flow cytometry and confocal microscopy assessed mHSP70 expression in 2D and 3D tumor cell cultures.
  • Chitosan core nanoparticles (NPs) were conjugated with a model drug (allocolchicinoid).
  • Monoclonal antibodies (mAbs) against mHSP70 formed the shell of the final nanoparticles (CAD-NPs).

Main Results:

  • mHSP70 was detected on breast, pancreas, colon, and prostate cancer cells, as well as lymphomas.
  • Characterization confirmed NP size, charge, and functionality.
  • CAD-NPs exhibited enhanced toxicity against mHSP70-positive tumor cells compared to drug-loaded NPs without the antibody (CD-NPs).

Conclusions:

  • Monoclonal antibodies targeting mHSP70 can serve as effective targeting vectors in anti-tumor therapy.
  • mHSP70 is a viable target for developing targeted nanomedicine delivery systems.
  • The developed CAD-NPs demonstrate potential for selective cancer treatment.
Keywords:
3D culturesallocolchicinoidchitosan core–shell nanoparticlesmembrane-bound heat shock protein 70targeted therapytargeting tumor cells

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