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siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

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Related Experiment Video

Updated: Jun 4, 2025

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Bio-Inspired Polymeric Solid Lipid Nanoparticles for siRNA Delivery: Cytotoxicity and Cellular Uptake In Vitro.

Keelan Jagaran¹, Saffiya Habib¹, Moganavelli Singh¹

¹Nano-Gene and Drug Delivery Laboratory, Discipline of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.

|December 17, 2024

Summary

This study developed novel solid lipid nanoparticles (SLNs) for safe siRNA delivery, showing potential for treating neurodegenerative disorders like Parkinson's disease.

Keywords:

Ginkgo biloba biological gene delivery nanomedicine neurological disorders siRNA solid lipid nanoparticles

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Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

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Last Updated: Jun 4, 2025

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Area of Science:

Nanomedicine
Biotechnology
Neuroscience

Background:

Current treatments for neurodegenerative disorders are palliative.
Innovative drug delivery systems are needed for curative therapies.
Nanomedicine offers precise diagnosis and treatment with fewer side effects.

Purpose of the Study:

To investigate a solid lipid nanoparticle (SLNP) system for binding and delivering small interfering RNA (siRNA) in vitro.
To incorporate Ginkgo biloba extract (GBE) for enhanced biocompatibility and neuroprotection.
To functionalize SLNPs with Poly-L-lysine (PLL) for improved siRNA binding, transport, and nuclease protection.

Main Methods:

Formulation of SLNPs using sphingomyelin and cholesterol, with GBE and PLL.
Physicochemical characterization of SLNPs.
Assessment of siRNA binding and protection via agarose gel electrophoresis.
In vitro studies including cytotoxicity, apoptosis (Caspase 3/7 activity), and cellular uptake in SH-SY5Y and HEK293 cells.

Main Results:

GBE-PLL-SLNPs exhibited an average size of 93.2 nm.
Enhanced siRNA binding and protection from nuclease degradation were observed.
Minimal cytotoxicity (<10% in HEK293, <15% in SH-SY5Y) and reduced Caspase 3/7 activity were noted.
Efficient cellular uptake of the GBE-PLL-SLNPs was confirmed.

Conclusions:

The developed GBE-PLL-SLNP system demonstrates a promising proof of principle for in vitro therapeutic applications.
This nanomedicine approach shows potential for addressing unmet medical needs in neurological disorders.
Further research is warranted to explore the therapeutic efficacy of this system in vivo.