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Characterization and Safety Evaluation of Autoclaved Gut Commensal Parabacteroides goldsteinii RV-01
Tzu-Lung Lin1, Wan-Jiun Chen2, Chien-Min Hung2
1REVIVEBIO Co., Taipei 115, Taiwan.
International Journal of Molecular Sciences
|December 17, 2024
Summary
A novel strain of P. goldsteinii, RV-01, was isolated and found safe for food use. Autoclaved P. goldsteinii RV-01 demonstrated anti-inflammatory effects and passed all toxicity tests, supporting its use as a functional food ingredient.
Area of Science:
- Microbiology
- Gastroenterology
- Food Science
Background:
- Gut commensals are crucial for human health homeostasis.
- P. goldsteinii, influenced by prebiotics, shows potential in ameliorating chronic inflammatory diseases.
- Development as a next-generation probiotic (NGP) requires rigorous safety assessment for food ingredient use.
Purpose of the Study:
- To isolate and characterize a novel P. goldsteinii strain, RV-01, for potential use as a functional food ingredient.
- To assess the safety and efficacy of autoclaved P. goldsteinii RV-01.
Main Methods:
- Genomic and phenotypic characterization of P. goldsteinii RV-01.
- Bioinformatic analysis for virulence and beneficial genes.
- In vitro assessment of anti-inflammatory effects in human colon epithelial cells.
- Nonclinical safety assessment including genotoxicity, 28-day subacute, and 90-day subchronic toxicity studies.
Main Results:
- P. goldsteinii RV-01 lacks potential virulence genes and possesses beneficial traits like short-chain fatty acid production and anti-inflammatory compounds.
- Autoclaved P. goldsteinii RV-01 maintained anti-inflammatory activity.
- All genotoxicity and animal toxicity studies yielded negative results for toxicity.
Conclusions:
- Autoclaved P. goldsteinii RV-01 is safe for consumption as a food ingredient.
- The strain exhibits beneficial properties, supporting its potential as a next-generation probiotic.
Keywords:
Parabacteroides goldsteiniibeneficial microorganismfood ingredientgut microbiotasafetytoxicityMore Related Videos
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