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Updated: Jun 4, 2025

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

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ADAM10 Expression by Ameloblasts Is Essential for Proper Enamel Formation.

Shifa Shahid¹, Yuanyuan Hu², Fatma Mohamed¹

¹Division of Biosciences, College of Dentistry, Ohio State University, 305 W, 12th Ave., Columbus, OH 43210, USA.

International Journal of Molecular Sciences

|December 17, 2024

Summary

This summary is machine-generated.

ADAM10 proteinase is essential for tooth enamel development. Its absence in ameloblasts causes softer, less dense enamel with disorganized structures, highlighting ADAM10

Keywords:

Cre Lox recombination ameloblast migration cervical enamel density hardness mouse occlusal enamel volume

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Published on: April 13, 2022

Area of Science:

Biochemistry
Developmental Biology
Oral Biology

Background:

ADAM10 is a proteinase known to cleave numerous substrates.
ADAM10 is expressed by ameloblasts, cells crucial for enamel formation.

Purpose of the Study:

To investigate the necessity of ADAM10 in ameloblasts for proper enamel development.

Main Methods:

Generated ADAM10 conditional knockout (cKO) mice by deleting Adam10 specifically in ameloblasts using the Amelx-iCre; Adam10 floxed mouse model.
Analyzed enamel characteristics, including density, volume, and rod structure, in the generated cKO mice.

Main Results:

ADAM10 cKO mice exhibited discolored, softer enamel with significantly reduced density and volume in incisors and molars.
Enamel rod patterns in incisors of cKO mice were progressively disorganized, featuring gaps and S-shaped rods.
ADAM10 cleaves key enamel formation proteins (RELT, COL17A1) and cell adhesion molecules (E-cadherin, N-cadherin) involved in ameloblast function.

Conclusions:

ADAM10 expression in ameloblasts is indispensable for normal enamel formation and structural integrity.
ADAM10's role in cleaving structural proteins and cell-cell contacts is critical for regulating ameloblast movement and enamel mineralization.