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A solid-phase radioimmunoassay for human corticosteroid binding globulin.

P A Robinson, M S Langley, G L Hammond

The Journal of Endocrinology

|February 1, 1985

Summary

A new radioimmunoassay (RIA) accurately measures human corticosteroid binding globulin (CBG) in serum. This method provides reliable CBG concentrations for various populations, including pregnant women.

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Area of Science:

Endocrinology
Immunochemistry
Analytical Chemistry

Background:

Corticosteroid Binding Globulin (CBG) plays a crucial role in regulating cortisol bioavailability.
Accurate measurement of serum CBG concentrations is essential for understanding endocrine function.
Existing methods may lack the specificity or sensitivity required for precise CBG quantification.

Purpose of the Study:

To develop and validate a radioimmunoassay (RIA) for quantifying human corticosteroid binding globulin (CBG).
To assess the accuracy, specificity, and precision of the developed RIA method.
To determine normal serum CBG concentrations in different demographic groups.

Main Methods:

Development of a RIA using 125I-labelled CBG and a solid-phase CBG-antiserum (CBG-Ab-cellulose).
Incubation of serum samples or CBG standards with labelled CBG and antibody-bound cellulose.
Measurement of bound radioactivity to quantify CBG concentrations.

Main Results:

The RIA demonstrated high specificity, confirmed by parallel displacement curves for various sera and CBG standards.
Excellent recovery (99 +/- 8%) and a strong correlation (r = 0.97) with cortisol binding capacity were observed.
Low intra-assay (<5%) and interassay (<9%) coefficients of variation indicate high precision.
Mean serum CBG concentrations varied across groups, notably higher in pregnant women (47.1 +/- 10.5 mg/l).

Conclusions:

A robust and validated RIA for human CBG has been successfully developed.
The method is accurate, specific, and precise for measuring serum CBG concentrations.
This RIA provides a valuable tool for clinical and research applications in endocrinology.

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