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Updated: Jun 4, 2025
Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
Published on: September 17, 2021
Relevance of oxidative stress for small intestinal injuries induced by nonsteroidal anti-inflammatory drugs: A
Yuki Baba1, Seiji Kawano2, Akinobu Takaki2
1Department of Internal Medicine, Japanese Red Cross Himeji Hospital, Himeji, Japan.
Abstract:
Several reports revealed that oxidative stress was involved in the mouse model of nonsteroidal anti-inflammatory drug (NSAIDs)-induced small intestinal mucosal injuries. Thus, we aimed to investigate in the prospective clinical study, that the relevance of oxidative stress balance in small intestinal mucosal injury in NSAIDs users. We prospectively included 60 patients who had been taking NSAIDs continuously for more than 3 months and exhibited obscure gastrointestinal bleeding (number UMIN 000011775). Small intestinal mucosal injuries were assessed by capsule endoscopy (CE), and reactive oxygen metabolites (d-ROMs) levels and oxidant capacity (OXY) adsorbent test were performed to investigate the relevance of oxidative stress balance. More than half of the patients (N = 32, 53%) had small intestinal mucosal injuries by CE, and 14 patients (24%) had ulcers. The incidence of ulcers was relatively higher in nonaspirin users. Serum OXY levels were significantly lower in the mucosal injury group (P = .02), and d-ROM levels were significantly higher in the ulcer group (P < .01). In aspirin users, d-ROM and OXY levels did not differ significantly with respect to mucosal injuries or ulcers. However, in nonaspirin users, OXY level was significantly lower in the mucosal injury group (P = .04), and d-ROM levels were significantly higher in the ulcer group (P = .02). Nonaspirin NSAIDs-induced intestinal mucosal injury is associated with antioxidant systems, resulting in increased oxidative stress.
Insights
Nonsteroidal anti-inflammatory drug (NSAID) use can cause small intestinal injuries. This study found that NSAID-induced intestinal damage is linked to an imbalance in oxidative stress, particularly in nonaspirin users.
Area of Science:
- Gastroenterology
- Biochemistry
- Clinical Medicine
Background:
- Oxidative stress is implicated in NSAID-induced small intestinal mucosal injuries in mouse models.
- Understanding this link in human NSAID users is crucial for clinical management.
Purpose of the Study:
- To prospectively investigate the role of oxidative stress balance in NSAID users with small intestinal mucosal injury.
- To differentiate the impact of aspirin versus nonaspirin NSAIDs on oxidative stress markers.
Main Methods:
- A prospective study included 60 patients on continuous NSAID use for over 3 months with obscure gastrointestinal bleeding.
- Small intestinal mucosal injuries were assessed using capsule endoscopy (CE).
- Oxidative stress was evaluated by measuring reactive oxygen metabolites (d-ROMs) and oxidant capacity (OXY) levels.
Main Results:
- 53% of patients showed small intestinal mucosal injuries on CE, with 24% having ulcers.
- Lower serum OXY levels and higher d-ROM levels were associated with mucosal injury and ulcers, respectively.
- Nonaspirin NSAID users exhibited significantly lower OXY levels and higher d-ROM levels in relation to mucosal injury and ulcers, unlike aspirin users.
Conclusions:
- Nonaspirin NSAID-induced intestinal mucosal injury is associated with an imbalance in the antioxidant system, leading to increased oxidative stress.
- Oxidative stress markers, d-ROMs and OXY, are relevant indicators in NSAID-associated small intestinal injuries.
- Findings suggest a differential impact of aspirin versus nonaspirin NSAIDs on oxidative stress and intestinal health.
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