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Furan impairs cell function by inducing oxidative stress, DNA damage and apoptosis in mouse Sertoli cells in vitro

Yasemin Aydin1, Yasemin Ulku Dikbasan2, Banu Orta-Yilmaz1

  • 1Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey.

Drug and Chemical Toxicology
|December 17, 2024
PubMed

Insights

Furan exposure damages male reproductive Sertoli cells, decreasing viability and inducing oxidative stress and apoptosis. This research highlights furan

Area of Science:

  • Toxicology
  • Reproductive Biology
  • Cell Biology

Background:

  • Furan is a heat-induced food contaminant with known systemic toxicity.
  • Sertoli cells are vital for male reproductive function.
  • The specific impact of furan on Sertoli cells remains uninvestigated.

Purpose of the Study:

  • To investigate the toxic effects of furan on TM4 Sertoli cells *in vitro*.
  • To determine furan's impact on Sertoli cell viability, oxidative stress, and apoptosis.

Main Methods:

  • TM4 Sertoli cells were exposed to varying concentrations of furan (750, 1500, 3000 µM) for 24 hours.
  • Cell viability, lactate dehydrogenase activity, malondialdehyde (MDA) levels, antioxidant enzyme activity, and apoptotic gene expression were assessed.

Main Results:

  • Furan exposure significantly decreased Sertoli cell viability and increased cytotoxicity, evidenced by elevated lactate dehydrogenase.
  • Furan induced oxidative stress, indicated by increased MDA levels and reduced antioxidant enzyme activities.
  • Furan triggered apoptosis in Sertoli cells through DNA damage and altered apoptotic gene expression.

Conclusions:

  • Furan causes significant cytotoxicity and oxidative damage to Sertoli cells.
  • Furan induces apoptosis in Sertoli cells, potentially leading to male infertility.
  • This study underscores the reproductive risks associated with furan exposure.
Keywords:
DNA damageFuranSertoli cellsapoptosiscytotoxicityoxidative stress

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