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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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Emerging technologies for single-cell glycomics.

Sunada Keisham1,2, Hiroaki Tateno1,2

  • 1Cellular and Molecular Biotechnology Research Institute, Multicellular System Regulation Research Group, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

BBA Advances
|December 17, 2024
PubMed
Summary
This summary is machine-generated.

Single-cell glycomics is now possible with scGR-seq, a new technology that converts glycan data into genetic information. This breakthrough allows simultaneous measurement of the glycome and transcriptome in thousands of single cells.

Keywords:
GlycanGlycomeGlycomicsSequencingSingle-cellscGR-seq

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Area of Science:

  • Biochemistry
  • Genomics
  • Cell Biology

Background:

  • Glycans on cell surfaces regulate intercellular communication.
  • Single-cell omics reveal cellular heterogeneity but glycan analysis remains challenging.
  • Glycan complexity and unamplifiable nature hinder single-cell glycomics.

Purpose of the Study:

  • To introduce a novel technology for single-cell glycomics.
  • To review recent advances in single-cell glycomics.
  • To highlight the capabilities of scGR-seq.

Main Methods:

  • Developed single-cell glycan and RNA sequencing (scGR-seq).
  • Converts glycan information into DNA barcodes using lectins.
  • Enables simultaneous glycome and transcriptome measurement via PCR and next-generation sequencing.

Main Results:

  • scGR-seq allows high-throughput single-cell glycomics.
  • The technology integrates glycan and RNA sequencing.
  • Enables comprehensive analysis of cellular heterogeneity.

Conclusions:

  • scGR-seq overcomes limitations in single-cell glycan analysis.
  • Provides a powerful tool for studying cell surface glycans.
  • Advances the field of single-cell multi-omics research.