Grazing-incidence X-ray diffraction elucidates structural correlations in fluid monolayers of lipids and surfactants

Biological membranes predominantly consist of fluid lipid phases featuring lateral mobility and a considerable disorder of their hydrocarbon chains. Langmuir monolayers of lipids at the air/water interface are versatile model systems for fundamental physicochemical and biophysical membrane investigations. Grazing-incidence X-ray diffraction (GIXD) is a powerful tool for the structural characterization of such monolayers but has so far been used almost exclusively for lipid phases of crystalline ordering giving rise to sharp diffraction peaks. Here, we use GIXD for the characterization of fluid monolayers of phospholipids and of water-soluble surfactants. We find that these layers feature spatiotemporally localized, structurally correlated hydrocarbon chain regions that involve only a few molecules and have only a small extension vertically. The abundance of these regions increases with increasing lateral packing density due to compression until the transition into an ordered phase occurs.