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Updated: Jun 4, 2025
Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
Protective Effects of Adipose Mesenchymal Stem Cell Secretome On Oxidative Stress-Induced Bisphenol-A in Isolated Rat
Maryam Zohour Soleimani1,2, Layasadat Khorsandi2,3, Yousef Asadi-Fard4
1Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Objective:
This study aimed to explore the potential protective effects of adipose-derived mesenchymal stem cell secretome (ASE) on oxidative stress triggered by Bisphenol-A (BisA) exposure in testicular mitochondria and sperm quality of rats.
Methods:
Testicular tissue mitochondria and sperms were exposed to BisA (8 μM) and ASE (50 or 100 μg). ∆Ψm (mitochondrial membrane potential), reactive oxygen species (ROS) levels, antioxidant biomarkers, and sperm parameters were measured.
Results:
BisA elevated biomarkers of oxidative stress in mitochondria, while the levels of antioxidant activity and ∆Ψm decreased significantly. BisA harmed the morphology, survival rate, and mobility of the spermatozoids. ASE lowered malondialdehyde contents and ROS generation in the mitochondria, increased ∆Ψm, and reversed sperm quality.
Conclusions:
These data indicated that ASE effectively reduced BisA-induced damage to mitochondria and enhanced sperm quality by averting oxidative stress.
