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Related Experiment Videos

Plasmids in Escherichia coli controlling citrate-utilizing ability.

N Ishiguro, C Oka, Y Hanzawa

    Applied and Environmental Microbiology
    |November 1, 1979
    PubMed
    Summary

    Citrate utilization genes from pig sewage Escherichia coli were transferred to E. coli K-12 via conjugation. These genes, often linked to drug resistance, were found on thermosensitive plasmids, with some strains harboring multiple types.

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    Corrigenda.

    The bone & joint journal·2021

    Area of Science:

    • Microbiology
    • Genetics
    • Environmental Science

    Background:

    • Citrate utilization is a key metabolic trait in bacteria.
    • Escherichia coli strains from environmental sources like pig sewage can harbor mobile genetic elements.
    • Understanding gene transfer mechanisms is crucial for microbial ecology and public health.

    Purpose of the Study:

    • To investigate the transfer of citrate utilization ability from environmental Escherichia coli strains to a laboratory strain.
    • To characterize the plasmids responsible for citrate utilization and their associated traits.
    • To determine the genetic elements involved in citrate metabolism in bacteria from agricultural environments.

    Main Methods:

    • Conjugation experiments were performed to transfer citrate utilization (Cit) ability.

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  • Thermosensitivity and incompatibility group analysis of plasmids were conducted.
  • Retransfer experiments and growth characterization on Simmons citrate agar were used to confirm gene transfer.
  • Plasmid characterization included determining their fertility inhibition (Fi) factor and compatibility with reference R plasmids.

    • Main Results:

    • Citrate utilization ability was successfully transferred from 19 out of 22 citrate-positive E. coli strains to E. coli K-12.
    • The conjugal transfer of Cit abilities was often thermosensitive and associated with drug resistance.
    • Sixteen of the 19 thermosensitive plasmids conferring citrate utilization and drug resistance belonged to incompatibility group H1 and were Fi-.
    • Two conjugative plasmids carrying only citrate utilization were Fi+ and compatible with reference R plasmids, suggesting coexistence of different plasmid types within strains.

    Conclusions:

    • Environmental E. coli strains possess plasmids that can transfer citrate utilization ability, often linked to drug resistance.
    • Thermosensitive H1 plasmids are significant carriers of both citrate utilization and drug resistance genes.
    • The coexistence of different types of conjugative plasmids within single strains highlights the complexity of horizontal gene transfer in environmental bacteria.