Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

23.0K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
23.0K
Replicative Cell Senescence02:15

Replicative Cell Senescence

3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.1K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.1K
Mitochondria01:37

Mitochondria

10.7K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
10.7K
Aging01:26

Aging

37
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
37
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

microRNA-25 drives immune checkpoint therapy resistance by repressing innate and humoral immunity via Syndecan-3.

Nature communications·2026
Same author

Telomerase RNA Shapes the Evolutionary Diversity of Telomerase Ribonucleoproteins (RNPs).

Cold Spring Harbor perspectives in biology·2025
Same author

Monkeyflower (Mimulus) uncovers the evolutionary basis of the eukaryote telomere sequence variation.

PLoS genetics·2025
Same author

A degenerate telomerase RNA directs telomeric DNA synthesis in lepidopteran insects.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Transposition, duplication, and divergence of the telomerase RNA underlies the evolution of <i>Mimulus</i> telomeres.

bioRxiv : the preprint server for biology·2023
Same author

Room-temperature structural studies of SARS-CoV-2 protein NendoU with an X-ray free-electron laser.

Structure (London, England : 1993)·2023
Same journal

Future Directions in Biotechnological and Pharmacological Applications of CAIs.

Sub-cellular biochemistry·2026
Same journal

Industrial and Environmental Applications of Carbonic Anhydrases.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Arthritis, Neuropathic Pain, Acute Mountain Sickness, and Cerebral Ischemia.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Neurological Disorders, Mechanisms and Therapeutic Potential.

Sub-cellular biochemistry·2026
Same journal

Carbonic Anhydrase Inhibitors in Oncology.

Sub-cellular biochemistry·2026
Same journal

Therapeutic Applications of Carbonic Anhydrase Inhibitors in Ophthalmology.

Sub-cellular biochemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2025

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
08:34

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Published on: April 13, 2015

10.3K

Telomerase-Mediated Anti-Ageing Interventions.

Phoebe L Dunn1, Dhenugen Logeswaran2, Julian J-L Chen3

  • 1School of Life Sciences, Arizona State University, Tempe, Arizona, USA.

Sub-Cellular Biochemistry
|December 18, 2024
PubMed
Summary
This summary is machine-generated.

Preventing telomere shortening may delay ageing. Strategies to increase telomerase levels or activity are explored, offering potential anti-ageing benefits but requiring careful risk-benefit analysis.

Keywords:
Cell senescenceDNA replicationEnd replication problemHayflick limitRibonucleoproteinTelomerase activationTelomere shortening

More Related Videos

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

46.4K
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Published on: September 2, 2014

14.6K

Related Experiment Videos

Last Updated: Jun 4, 2025

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
08:34

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Published on: April 13, 2015

10.3K
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

46.4K
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Published on: September 2, 2014

14.6K

Area of Science:

  • Cellular Biology
  • Genetics
  • Gerontology

Background:

  • Cellular ageing is linked to declining chromosome integrity and telomere shortening.
  • Telomeres, protective DNA caps, shorten with replication due to the end-replication problem.
  • Telomerase counteracts this shortening, maintaining telomere length in specific cells.

Purpose of the Study:

  • To review anti-ageing interventions targeting telomere shortening.
  • To analyze strategies that increase telomerase levels or activity.
  • To discuss the risks, benefits, and future of these interventions.

Main Methods:

  • Literature review of anti-ageing strategies.
  • Analysis of telomere biology and telomerase function.
  • Discussion of cellular senescence as a hallmark of ageing.

Main Results:

  • Progressive telomere shortening in somatic cells leads to cellular senescence.
  • Senescent cell accumulation contributes to organismal ageing.
  • Preventing telomere shortening is a potential anti-ageing approach.

Conclusions:

  • Increasing telomerase activity is a promising strategy to mitigate ageing.
  • Careful consideration of risks and benefits is essential for therapeutic development.
  • Further research is needed to optimize telomere-targeted anti-ageing interventions.