Related Experiment Video
Updated: Jun 28, 2026
Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
Published on: June 20, 2015
Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion
Ted S Acott1, Michael P Fautsch2, Weiming Mao3,4
1Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
Ex vivo eye models are crucial for understanding intraocular pressure (IOP) regulation in glaucoma. This study details various perfusion techniques for studying conventional outflow, vital for IOP management.
Area of Science:
- Ophthalmology
- Physiology
- Biomedical Engineering
Background:
- Elevated intraocular pressure (IOP) is the primary risk factor for glaucomatous optic neuropathy.
- IOP regulation is mainly influenced by conventional outflow, making its study critical for glaucoma treatment.
Purpose of the Study:
- To present detailed experimental protocols for ex vivo models used in studying IOP regulation.
- To outline the advantages and limitations of various ex vivo perfusion techniques for assessing outflow function.
Main Methods:
- Perfused whole globes
- Stationary anterior segment organ culture
- Perfused anterior segment organ culture (human and animal)
- Perfused human corneal rims
- Perfused human anterior segment wedges
Main Results:
- The study provides well-tested experimental details for multiple ex vivo models.
- Each method offers specific advantages for investigating IOP regulation and outflow pathways.
Conclusions:
- Ex vivo perfusion models are essential tools for advancing the understanding of IOP regulation.
- Careful consideration of each model's strengths and limitations is key to successful research in glaucoma.
More Related Videos
05:27Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice
Published on: August 13, 2020
06:26Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment
Published on: July 14, 2023
