Identification of potential substrates of LNX1 in chicken cells

Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin ligase, catalyzes the ubiquitination and subsequent degradation of specific substrate proteins. A cell line with stable knockdown of the LNX1 gene was developed using chicken macrophages (HD11) as the model. The relative quantitative values of each protein were analyzed using 4D-FastDIA quantitative proteomics. This identified 319 proteins with up-regulated expression, a majority of which relate to the immune system. Overall, this study uncovered potential substrate proteins targeted by LNX1 and their associated biological pathways. The findings suggest that LNX1 could be implicated in the regulation of the chicken innate immune system by mediating protein ubiquitination.