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Multistage microfluidic assisted Co-Delivery platform for dual-agent facile sequential encapsulation.

Shixin Li1, Bing Yang2, Liang Ye1

  • 1School of Traditional Chinese Pharmacy, China Pharmaceutical University, 211198 Nanjing, Jiangsu, PR China.

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V
|December 18, 2024
PubMed
Summary
This summary is machine-generated.

A new microfluidic platform enables sequential encapsulation of multiple drugs with different properties into hybrid nanoparticles (HNPs). This advanced nano-drug delivery system shows promise for improved anti-tumor therapies.

Keywords:
Co-deliveryMicrofluidic chipNanocrystalNanoprecipitationSequential encapsulation

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Area of Science:

  • Nanomedicine
  • Materials Science
  • Chemical Engineering

Background:

  • Integrating multiple therapeutic agents in one nano-drug carrier is challenging due to diverse physicochemical properties.
  • Effective anti-tumor therapies require advanced drug delivery systems capable of handling complex drug combinations.

Purpose of the Study:

  • To develop a novel multi-stage microfluidic platform for sequential co-encapsulation of drugs with disparate physicochemical properties.
  • To synthesize and characterize hybrid nanoparticles (HNPs) loaded with various drug combinations.

Main Methods:

  • Utilized a multi-stage microfluidic TrH chip for sequential nano-precipitation.
  • Synthesized hybrid nanoparticles (HNPs) loaded with paclitaxel (PTX)-simvastatin (SV), PTX-lenvatinib (LV), and SV-LV.
  • Characterized HNPs for structure, particle size, encapsulation efficiency, and drug loading efficiency.

Main Results:

  • Achieved core-shell structured HNPs with uniform particle size distribution, surpassing conventional methods.
  • Demonstrated nearly 100% encapsulation efficiency for dual-drug loading across various ratios.
  • Quantified high drug loading efficiencies for PTX-SV/HNPs (14.97%), PTX-LV/HNPs (16.58%), and SV-LV/HNPs (19.21%).
  • Confirmed sequential drug release (SV and PTX) from PTX-SV/HNPs in vitro.
  • Showcased superior cytotoxicity of PTX-SV/HNPs against HepG2 cells compared to individual drugs.

Conclusions:

  • The multi-stage microfluidic platform effectively co-encapsulates drugs with significant physicochemical differences.
  • Developed HNPs offer a robust strategy for advanced multi-drug delivery in nanomedicine.
  • This approach holds significant potential for enhancing anti-tumor therapies.