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  1. Home
  3. Innovative Transgenic Zebrafish Biosensor For Heavy Metal Detection.
  1. Home
  3. Innovative Transgenic Zebrafish Biosensor For Heavy Metal Detection.

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Innovative transgenic zebrafish biosensor for heavy metal detection.

H M L P B Herath1, Rohini M de Silva2, R S Dassanayake3

  • 1Centre for Advanced Materials and Devices (CAMD), Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka; School of Life and Environmental Sciences, Deakin University, Pigdons Road, Geelong, VIC, 3216, Australia; Department of Life Sciences, Faculty of Science, NSBM Green University, Homagama, Sri Lanka.

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|December 18, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed genetically engineered zebrafish to detect cadmium (Cd2+) and zinc (Zn2+) heavy metals. This novel biosensor accurately senses low concentrations, offering a promising tool for environmental monitoring.

Keywords:
BiosensorEnvironmental monitoringHeavy metal detectionSynthetic gene circuitTransgenic zebrafish

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Toxicology

Background:

  • Heavy metal contamination poses significant risks to ecosystems and human health.
  • Developing sensitive and specific biosensors is crucial for effective environmental monitoring.
  • Zebrafish offer a viable model organism for toxicity and biosensing studies.

Purpose of the Study:

  • To engineer zebrafish as biosensors for detecting cadmium (Cd2+) and zinc (Zn2+).
  • To evaluate the biosensor's sensitivity, specificity, and detection limits for heavy metals.
  • To assess the toxicity of heavy metals on zebrafish early developmental stages.

Main Methods:

  • Synthesized a novel heavy metal-sensitive gene construct (metallothionine 2 promoter with DsRed reporter gene).
  • Integrated the construct into zebrafish embryos using a Tol2 transposon transposase system.
  • Applied the transgenic zebrafish line for biosensing Cd2+ and Zn2+ and assessed toxicity via a modified OECD Fish Embryo Toxicity test.

Main Results:

  • The mt2-DsRed2 zebrafish biosensor demonstrated a linear correlation between heavy metal concentration and DsRed fluorescence for Cd2+ and Zn2+ (p < 0.01).
  • Achieved a minimum detection limit of 4 ppb for Cd2+ and Zn2+, with no cross-reactivity to Ni2+.
  • Cd2+, Zn2+, and Ni2+ showed no significant toxicity to zebrafish embryos at concentrations up to 10 ppb.

Conclusions:

  • The developed transgenic zebrafish line serves as an accurate and specific biosensor for low-concentration Cd2+ and Zn2+.
  • This biosensor is suitable for laboratory-based heavy metal assessment assays and environmental monitoring.
  • The findings confirm the biocompatibility of the transgenic zebrafish for heavy metal sensing applications.