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CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Related Experiment Video

Updated: Jun 4, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

CRISPR/Cas: a toolkit for plant disease diagnostics.

Yuanyuan Zhu¹, Xiaoping Yu², Jian Wu¹

¹College of Biosystems Engineering and Food Science & ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 310058/311215, China.

Trends in Plant Science

|December 18, 2024

Summary

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) technology shows promise for diagnosing plant diseases. This advancement aids in monitoring plant growth and preventing agricultural losses.

Keywords:

CRISPR/Cas nucleic acid imaging pathogens plant disease diagnostics

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Area of Science:

Plant pathology
Molecular biology
Agricultural science

Background:

Plant diseases caused by genetic factors and infectious pathogens significantly impact global agricultural production.
Early and accurate diagnosis is crucial for effective disease management and yield protection.

Purpose of the Study:

To review the advancements and potential applications of CRISPR/Cas technology in plant disease diagnostics.
To highlight the significance of CRISPR/Cas systems for monitoring plant health and preventing disease outbreaks.

Main Methods:

Review of recent scientific literature on CRISPR/Cas applications in plant pathology.
Analysis of the diagnostic capabilities of CRISPR/Cas systems for detecting plant pathogens and genetic abnormalities.

Main Results:

CRISPR/Cas systems offer high specificity and sensitivity for nucleic acid analysis.
Demonstrated potential for rapid and accurate detection of various plant diseases.

Conclusions:

CRISPR/Cas technology represents a significant breakthrough for plant disease diagnostics.
This technology is vital for enhancing agricultural monitoring and disease prevention strategies.