Phosphorus two-dimensional NMR spectroscopy of perfused rat hearts

The theory behind the technique of two-dimensional (2-D) NMR is presented with particular reference to 2-D J-spectroscopy and 2-D exchange spectroscopy. Experiments are described in which both these types of 2-D NMR are applied to the isolated perfused rat heart. Possible advantages of 2-D techniques and future applications to physiological systems are discussed.