Related Experiment Videos
Serial fluorometric documentation of fluorescein dye delivery
Surgery
|February 1, 1985
Summary
Fiberoptic fluorometry offers precise assessment of tissue viability and blood flow. This study confirms its reliability and repeatability for predicting skin flap outcomes, even with repeated measurements.
Area of Science:
- Biomedical Engineering
- Surgical Research
- Diagnostic Imaging
Background:
- Fiberoptic fluorometry, introduced in 1980, enhances the fluorescein test for blood flow and tissue viability assessment.
- Objective and precise methods are crucial for evaluating tissue perfusion.
Purpose of the Study:
- To determine the repeatability of fiberoptic fluorometry at close time intervals.
- To identify factors influencing the reliability of this technique for tissue viability prediction.
Main Methods:
- Four fluorescein dye injections were administered at varied doses and times.
- Skin flaps were scanned within 2 minutes post-injection (1.5 mg/kg dose).
- Repeatability was assessed using multiple injections (1.5, 3.0, 6.0 mg/kg) at 15-minute intervals.
Main Results:
- Fluorometric indices accurately predict skin flap viability.
- A 2-minute scan post-1.5 mg/kg fluorescein injection predicted flap fate with nearly 100% accuracy.
- Repeated injections at 15-minute intervals yielded consistent viability predictions.
Conclusions:
- Fiberoptic fluorometry is a highly repeatable technique for assessing tissue perfusion.
- It is valuable for monitoring perfusion status and changes due to interventions.
- This method offers precision in predicting skin flap outcomes.