Related Experiment Video

Updated: Jun 4, 2025

Analysis of SCAP N-glycosylation and Trafficking in Human Cells
11:27

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Published on: November 8, 2016

8.7K

SEC14L2 regulates the transport of cholesterol in non-small cell lung cancer through SCARB1

Qianhui Zhou1, Dianwu Li1, Yanchao Liang1

  • 1Department of Respiratory and Critical Care Medicine, Zhuzhou Central Hospital, No.116, Changjiang South Road, Tianyuan District, Zhuzhou, 412000, Hunan, China.

Lipids in Health and Disease
|December 19, 2024
PubMed
Abstract

Insights

Cholesterol metabolism is key in non-small cell lung cancer (NSCLC). The study found that SEC14L2 promotes NSCLC by increasing cholesterol uptake via SCARB1, inhibiting tumor growth when SEC14L2 is reduced.

Area of Science:

  • Oncology
  • Molecular Biology
  • Metabolic Research

Background:

  • Cholesterol metabolism inhibition shows promise for non-small cell lung cancer (NSCLC) treatment.
  • The precise role of SEC14L2 in NSCLC lipid metabolism regulation is not well understood.
  • This research explores cholesterol metabolism-related genes' impact on NSCLC progression.

Purpose of the Study:

  • To investigate the role of SEC14L2 in NSCLC progression.
  • To elucidate the mechanism by which SEC14L2 influences cholesterol metabolism in NSCLC.
  • To identify SEC14L2 as a potential therapeutic target in NSCLC.

Main Methods:

  • Survival analysis and Cox regression identified SEC14L2 as a prognostic marker.
  • Cell proliferation and migration assays (CCK-8, EdU, colony formation, wound-healing) were performed.
  • Immunofluorescence, Co-IP, and cholesterol depletion/rescue experiments assessed SEC14L2 function and its interaction with SCARB1.

Main Results:

  • SEC14L2 is highly expressed in NSCLC cells and correlates with poor prognosis.
  • SEC14L2 knockdown significantly inhibited NSCLC cell proliferation, migration, and tumor growth.
  • SEC14L2 was found to promote cholesterol uptake in NSCLC cells by interacting with and up-regulating SCARB1.

Conclusions:

  • SEC14L2 plays a crucial role in promoting NSCLC development.
  • SEC14L2 enhances NSCLC progression by up-regulating SCARB1 expression, leading to increased cholesterol uptake.
  • Targeting SEC14L2 may offer a novel therapeutic strategy for NSCLC.
Keywords:
Cholesterol metabolismNSCLCSCARB1SEC14L2

More Related Videos

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

13.3K
The Soft Agar Colony Formation Assay08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

111.4K

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
501
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.3K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K