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Distinct roles of centriole distal appendage proteins in ciliary assembly and disassembly

Su-Yeon Je1,2, Hyuk Wan Ko3,4,5

  • 1Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea.

Cell Communication and Signaling : CCS
|December 19, 2024
PubMed

Insights

Distal appendage proteins regulate primary cilium assembly and disassembly. Two subgroups were identified: one for assembly (CEP83, SCLT1, CEP164) and another for disassembly (CEP89, FBF1), impacting cell cycle signaling.

Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • The primary cilium is a crucial organelle involved in cell signaling and development.
  • The centriole distal appendage (DA) is vital for initiating ciliogenesis by anchoring the mother centriole.
  • The specific roles of DA proteins in ciliary dynamics remain largely unknown.

Purpose of the Study:

  • To elucidate the precise functions of distal appendage proteins in primary cilium assembly and disassembly.
  • To functionally categorize distal appendage proteins based on their roles in ciliary dynamics.

Main Methods:

  • Utilized siRNA-mediated knockdown to assess the function of distal appendage proteins.
  • Analyzed ciliary assembly and disassembly kinetics in mutant and knockdown cells.
  • Investigated the impact on the Aurora A kinase signaling pathway.

Main Results:

  • Cep89 mutant cells showed normal ciliogenesis but delayed ciliary disassembly.
  • DA proteins were functionally divided into two groups: CEP83, SCLT1, and CEP164 for assembly/docking, and CEP89 and FBF1 for disassembly.
  • CEP89 and FBF1 depletion impaired disassembly and disrupted Aurora A kinase localization and signaling.

Conclusions:

  • Distal appendage proteins form functional modules for distinct aspects of ciliary dynamics.
  • CEP89 and FBF1 play a specific role in regulating ciliary disassembly and Aurora A kinase signaling.
  • These findings have implications for understanding cellular signaling, homeostasis, and development.

Keywords:
Aurora ACEP89Ciliary disassemblyCiliogenesisDistal appendagePrimary cilium

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