Unravelling complex mechanisms in materials processes with cryogenic electron microscopy

Investigating nanoscale structural variations, including heterogeneities, defects, and interfacial characteristics, is crucial for gaining insight into material properties and functionalities. Cryogenic electron microscopy (cryo-EM) is developing as a powerful tool in materials science particularly for non-invasively understanding nanoscale structures of materials. These advancements bring us closer to the ultimate goal of correlating nanoscale structures to bulk functional outcomes. However, while understanding mechanisms from structural information requires analysis that closely mimics operation conditions, current challenges in cryo-EM imaging and sample preparation hinder the extraction of detailed mechanistic insights. In this Perspective, we discuss the innovative strategies and the potential for using cryo-EM for revealing mechanisms in materials science, with examples from high-resolution imaging, correlative elemental analysis, and three-dimensional and time-resolved analysis. Furthermore, we propose improvements in cryo-sample preparation, optimized instrumentation setup for imaging, and data interpretation techniques to enable the wider use of cryo-EM and achieve deeper context into materials to bridge structural observations with mechanistic understanding.