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Published on: October 4, 2019
CircMIB1 inhibits glioma development and progression through a competing endogenous RNA interaction network
Simin Chen1, Longping Li1, Wei Xu1
1Department of Clinical Laboratory, Yiyang Central Hospital, Yiyang, Hunan, China.
Introduction:
The critical role of circular RNAs as non-coding RNAs in glioma has been extensively investigated. Therefore, we aimed to explore the role and potential molecular mechanisms of circRNA-mind bomb homolog 1 (circMIB1) in gliomas.
Methods:
RNA sequencing was used to analyze the expression profiles of circRNAs in glioma tissues and normal brain tissues. Quantitative real-time polymerase chain reaction was implemented to examine the levels of circMIB1 in glioma cells and tissues. The circMIB1 was identified as a cyclic RNA molecule by DNA nucleic acid electrophoresis and ribonuclease R assay. The relationship between circMIB1 expression and the prognosis of glioma patients and its potential as a biomarker were analysed using Kaplan-Meier, Receiver operating characteristic curves, and Principal component analysis. Bioinformatics analysis predicted the miRNAs that bind to circMIB1 and their downstream targets, and analysed the functions of these genes.
Results:
Firstly, a novel circRNA molecule termed circMIB1 was identified and validated by RNA sequencing. The expression of circMIB1 was significantly downregulated in glioma cells and tissues, and was closely associated with the tumor grade and survival prognosis of patients with glioma. Hence, it may be useful as a biomarker for glioma. Secondly, it was predicted that circMIB1 binds to hsa-miR-1290 based on bioinformatics analysis, which was significantly upregulated in glioma cells and tissues, and correlated with the tumor grade and overall survival of patients. Thirdly, through a series of bioinformatics analyses identified six genes downstream of hsa-miR-1290 that were significantly associated with glioma expression and prognosis, these genes are associated with cell cycle, cell necrosis and cell circadian rhythms.
Discussion:
CircMIB1 may play a role in inhibiting glioma development through the hsa-miR-1290 competitive endogenous RNA interaction network, these findings provide new ideas and directions for the diagnosis and treatment of glioma.
Insights
Circular RNA circMIB1 is downregulated in glioma and may inhibit tumor development. This finding suggests circMIB1 could be a potential biomarker for glioma diagnosis and treatment.
Area of Science:
- * Molecular biology
- * Oncology
- * Genetics
Background:
- * Circular RNAs (circRNAs) are crucial non-coding RNAs with emerging roles in various cancers.
- * Glioma, a primary brain tumor, presents complex challenges in diagnosis and treatment.
- * Understanding the specific functions of circRNAs in glioma is essential for developing novel therapeutic strategies.
Purpose of the Study:
- * To investigate the role and molecular mechanisms of circRNA-mind bomb homolog 1 (circMIB1) in glioma.
- * To determine if circMIB1 can serve as a diagnostic biomarker for glioma.
- * To elucidate the regulatory network involving circMIB1 in glioma progression.
Main Methods:
- * RNA sequencing to identify differentially expressed circRNAs in glioma tissues.
- * Quantitative real-time PCR to validate circMIB1 expression levels.
- * Bioinformatics analysis to predict miRNA targets and downstream genes of circMIB1.
- * Kaplan-Meier and ROC curve analyses to assess circMIB1 as a prognostic biomarker.
Main Results:
- * circMIB1 was identified as a novel circRNA and found to be significantly downregulated in glioma tissues and cells.
- * Lower circMIB1 expression correlated with higher tumor grade and poorer prognosis in glioma patients.
- * circMIB1 was predicted to sponge hsa-miR-1290, which was upregulated in glioma and associated with poor survival.
- * Six downstream genes of hsa-miR-1290 involved in cell cycle and necrosis were identified.
Conclusions:
- * circMIB1 may function as a tumor suppressor in glioma by interacting with the hsa-miR-1290/target gene network.
- * circMIB1 holds potential as a diagnostic and prognostic biomarker for glioma.
- * These findings offer new insights into glioma pathogenesis and potential therapeutic targets.
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